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| Saturday 9 Jul 2016 | Home | Preston Athletic v Leith Athletic | Pre-Season Friendly | 3G Middleshot, Prestonpans | 0-2 | |
| Wednesday 20 Jul 2016 | Home | Preston Athletic v Dunbar Utd | Pre-Season Friendly | 3G Forester’s Park, Tranent | 1-3 | |
| Saturday 23 July 2016 | Home | Cowdenbeath XI v Arniston Rangers | Summer Tournament | Pennypit Park, Prestonpans | 2-5 | |
| Saturday 23 July 2016 | Home | Preston Athletic v Easthouses Lily | Summer Tournament | Pennypit Park, Prestonpans | 0-2 | |
| Sunday 24 July 2016 | Home | Cowdenbeath XI v Preston Athletic | Summer Tournament | Pennypit Park, Prestonpans | 6-1 | |
| Sunday 24 July 2016 | Home | Arniston Rangers v Easthouses Lily | Summer Tournament | Pennypit Park, Prestonpans | 3-0 | |
| Tuesday 26 Jul 2016 | Home | Preston Athletic v Newtongrange Star | Pre-Season Friendly | Pennypit Park, Prestonpans | 1-9 | |
| Saturday 30 July 2016 | Away | Selkirk v Preston Athletic | Lowland League | Yarrow Park, Selkirk | 0-3 | |
| Wednesday 3 Aug 2016 | Away | Tollcross Thistle v Preston Athletic | Friendly | Peffermill 3G, Edinburgh | ||
| Saturday 6 Aug 2016 | Home | Preston Athletic v BSC Glasgow | Lowland League | Pennypit Park, Prestonpans | 1-1 | |
| Wednesday 10 Aug 2016 | Away | Cumbernauld Colts v Preston Athletic | Lowland League | Broadwood Stadium, Cumbernauld | 5-0 | |
| Saturday 13 Aug 2016 | Home | Preston Athletic v Burntisland Shipyard | East of Scotland Qualifying Cup R1 | Pennypit Park, Prestonpans | 3-2 | |
| Saturday 20 Aug 2016 | Home | Preston Athletic v East Kilbride | Lowland League | Pennypit Park, Prestonpans | 0-5 | |
| Monday 22 Aug 2016 | Home | Preston Athletic v Edinburgh United JFC | Friendly | Pennypit Park, Prestonpans | 4-4 | |
| Saturday 27 Aug 2016 | Away | Hawick Royal Albert v Preston Athletic | Lowland League | Albert Park, Hawick | 3-0 | |
| Wednesday 31 Aug 2016 | Home | Preston Athletic v Vale of Leithen | Lowland League | Pennypit Park, Prestonpans | 0-3 | |
| Saturday 3 Sept 2016 | Home | Preston Athletic v Whitehill Welfare | Lowland League | Pennypit Park, Prestonpans | 0-2 | |
| Saturday 10 Sept 2016 | Away | Gala Fairydean Rovers v Preston Athletic | Lowland League | Netherdale 3G, Galashiels | 3-1 | |
| Saturday 17 Sept 2016 | Home | Preston Athletic v Tweedmouth Rangers | South Challenge Cup R1 | Pennypit Park, Prestonpans | 7-2 | |
| Saturday 24 Sept 2016 | Away | Nairn County v Preston Athletic | William Hill Scottish Cup R1 | Station Park, Nairn | 2-3 | |
| Saturday 1 Oct 2016 | Home | Preston Athletic v University of Stirling | Lowland League | Pennypit Park, Prestonpans | 4-3 | |
| Tuesday 4 Oct 2016 | Home | Preston Athletic v Dalbeattie Star | Lowland League | Pennypit Park, Prestonpans | 1-4 | |
| Saturday 8 Oct 2016 | Away | Civil Service Strollers v Preston Athletic | Lowland League | Marine Drive, Edinburgh | 3-1 | |
| Saturday 15 Oct 2016 | Home | Preston Athletic v Lothian Thistle HV | South Challenge Cup R2 | Pennypit Park, Prestonpans | 1-2 | |
| Saturday 22 Oct 2016 | Home | Preston Athletic v Montrose | William Hill Scottish Cup R2 | Pennypit Park, Prestonpans | 0-3 | |
| Saturday 29 Oct 2016 | Home | Preston Athletic v Leith Athletic | East of Scotland Qualifying Cup R2 | Pennypit Park, Prestonpans | 1-2 | |
| Saturday 05 Nov 2016 | Home | Preston Athletic v Edinburgh University | Lowland League | Pennypit Park, Prestonpans | 0-3 | |
| Saturday 12 Nov 2016 | Away | Gretna 2008 v Preston Athletic | Lowland League | Raydale Park, Gretna | 5-3 | |
| Tuesday 15 Nov 2016 | Away | Spartans v Preston Athletic | Lowland League | Ainslie Park 3G, Edinburgh | 2-1 | |
| Saturday 19 Nov 2016 | Away | East Stirlingshire v Preston Athletic | Lowland League | Ochilview, Larbert | 6-2 | |
| Saturday 26 Nov 2016 | Away | BSC Glasgow v Preston Athletic | Lowland League | Indodrill Stadium, Alloa | 8-3 | |
| Saturday 3 Dec 2016 | FREE WEEK | |||||
| Saturday 10 Dec 2016 | Home | Preston Athletic v Hawick Royal Albert | Lowland League | Pennypit Park, Prestonpans | 2-1 | |
| Saturday 17 Dec 2016 | Away | Dalbeattie Star v Preston Athletic | Lowland League | Islecroft, Dalbeattie | 2-3 | |
| Wednesday 21 Dec 2016 | Home | Preston Athletic v Coldstream | Friendly | 3G Middleshot, Prestonpans | 0-2 | |
| Saturday 31 Dec 2016 | Home | Preston Athletic v Civil Service Strollers | Lowland League | Pennypit Park, Prestonpans | 3-4 | |
| Saturday 7 Jan 2017 | Home | Preston Athletic v Spartans | Lowland League | Pennypit Park, Prestonpans | 0-2 | |
| Saturday 14 Jan 2017 | Away | Whitehill Welfare v Preston Athletic | Lowland League | Ferguson Park, Rosewell | P-P | |
| Saturday 21 Jan 2017 | FREE WEEK | William Hill Scottish Cup R4 East of Scotland Qualifying Cup Semi-Finals |
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| Saturday 28 Jan 2017 | Home | Preston Athletic v Gala Fairydean Rovers | Lowland League | Pennypit Park, Prestonpans | 3-4 | |
| Saturday 4 Feb 2017 | Home | Preston Athletic v East Stirlingshire | Lowland League | Pennypit Park, Prestonpans | P-P | |
| Wednesday 8 Feb 2017 | Away | Edinburgh Utd v Preston Athletic | Friendly | Dalkeith Campus | 1-3 | |
| Saturday 11 Feb 2017 | Away | East Kilbride v Preston Athletic | Lowland League | K Park, East Kilbride | 4-0 | |
| Saturday 18 Feb 2017 | Away | Vale of Leithen v Preston Athletic | Lowland League | Victoria Park, Innerleithen | 3-1 | |
| Saturday 25 Feb 2017 | Home | Preston Athletic v Gretna 2008 | Lowland League | Pennypit Park, Prestonpans | P-P | |
| Saturday 4 Mar 2017 | Away | University of Stirling v Preston Athletic | Lowland League | The Falkirk Stadium, Grangemouth | 5-3 | |
| Saturday 11 Mar 2017 | Home | Preston Athletic v Gretna 2008 | Lowland League | Pennypit Park, Prestonpans | 1-2 | |
| Saturday 18 Mar 2017 | Away | Whitehill Welfare v Preston Athletic | Lowland League | Ferguson Park, Rosewell | 4-1 | |
| Sunday 19 Mar 2017 | Spartans v Lothian Thistle HV | Football Nation East of Scotland Qualifying Cup Final | Oriam Indoor Pitch, Riccarton | 2-1 | ||
| Friday 24 Mar 2017 | Home | Preston Athletic v Cumbernauld Colts | Lowland League (Groundhop) | Pennypit Park, Prestonpans | 0-2 | |
| Saturday 1 Apr 2017 | Home | Preston Athletic v East Stirlingshire | Lowland League | Pennypit Park, Prestonpans | 1-7 | |
| Saturday 8 Apr 2017 | FREE WEEK | |||||
| Saturday 15 Apr 2017 | Home | Preston Athletic v Selkirk | Lowland League | Pennypit Park, Prestonpans | 3-2 | |
| Saturday 22 Apr 2017 | Away | Edinburgh University v Preston Athletic | Lowland League | East Peffermill, Edinburgh | 4-0 | |
| Saturday 29 Apr 2017 | Away | Hawick Royal Albert v Preston Athletic | Lowland League Cup R1 | Albert Park, Hawick | 2-4 | |
| Saturday 6 May 2017 | Away | Civil Service Strollers v Preston Athletic | Lowland League Cup R2 | Christie Gillies Park, Edinburgh | 6-2 | |
| Saturday 13 May 2017 | Lowland League Cup Semi-Finals SPFL League 2 Pyramid Play-Off |
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| Saturday 20 May 2017 | SPFL League 2 Pyramid Play-Off | |||||
| Sunday 21 May 2017 | Lowland League Cup Final | |||||
| Saturday 27 May 2017 | William Hill Scottish Cup Final | |||||
| Sunday 28 May 2017 | South Challenge Cup Final |