| Sat 10 Oct 2020 | Home | Preston Athletic v Kinnoull | League Conference B | Pennypit Park, Prestonpans | 0-5 |
| Tues 13 Oct 2020 | Home | Preston Athletic v Peebles Rovers | League Conference B | Pennypit Park, Prestonpans | 0-0 |
| Sat 17 Oct 2020 | Away | Luncarty v Preston Athletic | League Conference B | Brownfield Park, Luncarty | 1-0 |
| Sat 24 Oct 2020 | Home | Preston Athletic v Glenrothes | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Tue 27 Oct 2020 | Home | Preston Athletic v Eyemouth Utd | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 31 Oct 2020 | Away | Lochore Welfare v Preston Athletic | League Conference B | Central Park, Lochore | P-P |
| Sat 7 Nov 2021 | Home | Preston Athletic v Stirling University | League Conference B | Pennypit Park, Prestonpans | 1-1 |
| Tue 10 Nov 2020 | Home | Preston Athletic v Eyemouth Utd | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 7-0 |
| Sat 14 Nov 2020 | Away | Heriot Watt University v Preston Athletic | League Conference B | Riccarton 3G | 2-0 |
| Sat 21 Nov 2020 | Away | Troon v Preston Athletic | Challenge Cup R2 | Portland Park, Troon, KA10 6QN | 3-4 |
| Sat 28 November 2020 | Home | Preston Athletic v Oakley Utd | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-1 |
| Sat 5 Dec 2020 | Away | Newburgh Juniors v Preston Athletic | League Conference B | East Shore Park, Newburgh | P-P |
| Sat 12 Dec 2020 | Home | Preston Athletic v Hawick Royal Albert Utd | Scottish Cup Pre-Lim R2 | Pennypit Park, Prestonpans | P-P |
| Wed 16 Dec 2020 | Home | Preston Athletic v Hawick Royal Albert Utd | Scottish Cup Pre-Lim R2 | Pennypit Park, Prestonpans | 3-2 |
| Sat 19 Dec 2020 | Away | Burntisland Shipyard v Preston Athletic | League Conference B | Recreation Park, Burntisland | 4-1 |
| Sat 26 Dec 2020 | Away | Stenhousemuir v Preston Athletic | Scottish Cup R1 | Ochilview Park, Stenhousemuire, FK5 4QL | 4-1 |
| Sat 2 Jan 2021 | Away | Easthouses Lily v Preston Athletic | League Conference B | Newbattle Complex, Easthouses | P-P |
| Sat 9 Jan 2021 | Home | Preston Athletic v Heriot Watt University | League Conference B | Pennypit Park, Prestonpans, EH32 0BN | P-P |
| Sat 16 Jan 2021 | FREE WEEK | ||||
| Sat 23 Jan 2021 | Away | Lochgelly Albert v Preston Athletic | League Conference B | Gardiners Park, Lochgelly | P-P |
| Sat 30 Jan 2021 | Home | Preston Athletic v Burntisland Shipyard | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 6 Feb 2021 | Away | Kirkcaldy & Dysart v Preston Athletic | Challenge Cup R3 | Alex Penman Park, Den Road, Kirkcaldy, KY1 2ER | P-P |
| Sat 13 Feb 2021 | FREE WEEK | ||||
| Sat 20 Feb 2021 | Away | Kinnoull v Preston Athletic | League Conference B | Tulloch Park, Kinnoull | P-P |
| Sat 27 Feb 2021 | Home | Preston Athletic v Luncarty | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 6 Mar 2021 | Home | Preston Athletic v Edinburgh South | King Cup R1 | Pennypit Park, Prestonpans, EH32 9BN | 2:30 |
| Sat 13 Mar 2021 | Away | Glenrothes v Preston Athletic | League Conference B | Warout Stadium, Glenrothes | 2:30 |
| Sat 20 Mar 2021 | Home | Preston Athletic v Lochore Welfare | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2:30 |
| Sat 27 Mar 2021 | Home | Preston Athletic v Easthouses Lily | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2:30 |
| Sat 3 Apr 2021 | Home | Preston Athletic/Edin South v Ormiston/Oakley | King Cup R2 | Pennypit Park, Prestonpans | 2:30 |
| Sat 10 Apr 2021 | Away | Stirling University v Preston Athletic | League Conference B | Gannochy Sports Centre, Stirling Uni | 2:30 |
| Sat 17 Apr 2021 | Away | Oakley Utd v Preston Athletic | League Conference B | Blairwood Park, Oakley | 2:30 |
| Sat 24 Apr 2021 | Challenge Cup R6 / King Cup R3 | ||||
| Sat 1 May 2021 | Home | Preston Athletic v Newburgh Juniors | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2:30 |
| Sun 9 May 2021 | Away | Hawick Royal Albert v Preston Athletic | League Conference B | Albert Park, Hawick | 2:30 |
| Sat 15 May 2021 | Away | Eyemouth Utd v Preston Athletic | League Conference B | Warner Park, Eyemouth | 2:30 |
| Sat 22 May 2021 | Home | Preston Athletic v Lochgelly Albert | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2:30 |
| Sat 29 May 2021 | Away | Peebles Rovers v Preston Athletic | League Conference B | Whitestone Park, Peebles | 2:30 |