| Sat 17 July 2021 | Away | Kinnoull v Preston Athletic | League Conference B | Tulloch Park, Tulloch Road, Perth, PH1 2SN | 1-3 |
| Tues 20 July 2021 | Home | Preston Athletic v Lochore Welfare | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-0 |
| Sat 24 July 2021 | Home | Preston Athletic v Glenrothes | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 0-0 |
| Tue 27 July 2021 | Home | Preston Athletic v Heriot Watt University | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-0 |
| Sat 31 July 2021 | FREE WEEK – NO FIXTURE | ||||
| Sat 7 Aug 2021 | Home | Preston Athletic v Burntisland Shipyard | King Cup R1 | Pennypit Park, Prestonpans, EH32 9BN | 2-3 |
| Tue 10 Aug 2021 | Home | Preston Athletic v Burntisland Shipyard | East of Scotland Qualifying Cup | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 14 Aug 2021 | Away | Oakley Utd v Preston Athletic | League Conference B | Blairwood Park, Oakley, KY12 9QG | 0-2 |
| Tue 17 Aug 2021 | Home | Preston Athletic v Edinburgh South | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-2 |
| Sat 21 Aug 2021 | Away | Burntisland Shipyard v Preston Athletic | League Conference B | Recreation Park, Burntisland, KY3 0JG | 0-2 |
| Sat 28 Aug 2021 | Home | Preston Athletic v Threave Rovers | Scottish Cup Pre-Lim Round | Pennypit Park, Prestonpans, EH32 9BN | 2-0 |
| Sat 4 Sept 2021 | Home | Preston Athletic v Easthouses Lily | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 5-0 |
| Sat 11 Sept 2021 | Home | Preston Athletic v Fauldhouse Utd | South Challenge Cup R1 | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 18 Sept 2021 | Away | Lossiemouth v Preston Athletic | Scottish Cup R1 | Grant Park, Lossiemouth | 0-3 |
| Sat 25 Sept 2021 | Away | Lochgelly Albert v Preston Athletic | League Conference B | Gardiner’s Park, Lochgelly, KY5 9LJ | 0-5 |
| Sat 2 Oct 2021 | Home | Preston Athletic v Stirling University | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2-0 |
| Sat 9 Oct 2021 | Away | Hawick Royal Albert v Preston Athletic | League Conference B | Albert Park, Hawick, TD9 8AW | 0-4 |
| Sat 16 Oct 2021 | Home | Preston Athletic v Peebles Rovers | South Challenge Cup R2 | Pennypit Park, Prestonpans, EH32 9BN | 2-1 |
| Sat 23 Oct 2021 | Home | Preston Athletic v Auchinleck Talbot | Scottish Cup R2 | Pennypit Park, Prestonpans, EH32 9BN | 0-2 |
| Sat 30 Oct 2021 | Home | Preston Athletic v Luncarty | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 6 Nov 2021 | Away | Stirling University v Preston Athletic | League Conference B | Gannochy Sports Centre, Stirling, | 1-2 |
| Sat 13 Nov 2021 | Home | Preston Athletic v Peebles Rovers | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 4-0 |
| Sat 20 Nov 2021 | Away | Sauchie v Preston Athletic | South Challenge Cup R3 | Beechwood Park, Sauchie | 3-2 |
| Sat 27 Nov 2021 | Home | Preston Athletic v Kinnoull | East of Scotland Qualifying Cup R3 | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 4 Dec 2021 | Home | Preston Athletic v Kinnoull | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 11 Dec 2021 | Away | Lochore Welfare v Preston Athletic | League Conference B | Central Park, Crosshill, KY5 8AN | P-P |
| Sat 18 Dec 2021 | Home | Preston Athletic v Burntisland Shipyard | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| CHRISTMAS BREAK | |||||
| Wed 5 Jan 2022 | Away | Easthouses Lily v Preston Athletic | League Conference B | Newbattle Complex, Easthouses, EH22 4ST | P-P |
| Sat 8 Jan 2022 | Home | Preston Athletic v Hawick Royal Albert Utd | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 8-0 |
| Sat 15 Jan 2022 | Away | Peebles Rovers v Preston Athletic | League Conference B | Whitestone Park, Peebles | 0-1 |
| Sat 22 Jan 2022 | Home | Preston Athletic v Kinnoull | East of Scotland Qualifying Cup R3 | Pennypit Park, Prestonpans, EH32 9BN | 0-1 |
| Wed 2 Feb 2022 | Away | Edinburgh South v Preston Athletic | League Conference B | King’s Park, Dalkeith, EH22 3BA | P-P |
| Sat 5 Feb 2022 | Home | Preston Athletic v Luncarty | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-0 |
| Sat 12 Feb 2022 | Away | Luncarty v Preston Athletic | League Conference B | Brownlands Park, Luncarty, PH1 3EP | P-P |
| Sat 19 Feb 2022 | Home | Preston Athletic v Oakley Utd | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-2 |
| Sat 26 Feb 2022 | Home | Preston Athletic v Burntisland Shipyard | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2-3 |
| Sat 5 Mar 2022 | Away | Edinburgh South v Preston Athletic | League Conference B | King’s Park, Dalkeith | 1-2 |
| Sat 12 Mar 2022 | Home | Preston Athletic v Bathgate Thistle | League Cup (Qualifying) | Pennypit Park, Prestonpans, EH32 9BN | 5-0 |
| Sat 19 Mar 2022 | Away | Dunipace v Preston Athletic | League Cup (Qualifying) | Westfield Park, Denny | 1-1 |
| Fri 25 Mar 2022 | Away | Lothian Thistle HV v Preston Athletic | League Cup (Qualifying) | Ainslie Park 3G, Edinburgh | 1-0 |
| Sat 2 Apr 2022 | Home | Preston Athletic v University of Stirling | League Cup (Qualifying) | Pennypit Park, Prestonpans, EH32 9BN | 1-0 |
| Tue 5 Apr 2022 | Home | Preston Athletic v Kinnoull | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Sat 9 Apr 2022 | Away | Newburgh v Preston Athletic | League Conference B | East Shore Park, Newburgh | 0-6 |
| Sat 16 Apr 2022 | Home | Preston Athletic v Newburgh | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2-0 |
| Tue 19 Apr 2022 | Home | Preston Athletic v Kinnoull | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2-2 |
| Sat 23 Apr 2022 | Away | Glenrothes v Preston Athletic | League Conference B | Warout Stadium, Glenrothes, KY7 4JY | 1-1 |
| Sat 30 Apr 2022 | Away | Lochore Welfare v Preston Athletic | League Conference B | Central Park, Crosshill, Fife, KY5 8AT | 1-2 |
| Sat 7 May 2022 | Away | Heriot Watt University v Preston Athletic | League Conference B | John Brydson 3G, Riccarton, EH14 4AS | 1-0 |
| Wed 11 May 2022 | Away | Luncarty v Preston Athletic | League Conference B | Brownlands Park, Luncarty, PH1 3EP | 2-1 |
| Sat 14 May 2022 | Away | Easthouses Lily v Preston Athletic | League Conference B | Newbattle Complex, Easthouses, EH22 4ST | 0-3 |
| Sat 21 May 2022 | FREE WEEK – NO FIXTURE | ||||
| Sat 28 May 2022 | Home | Preston Athletic v Lochgelly Albert | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 2:30 |