| Fri 30 July 2021 | Home | Preston Athletic v Whitehill Welfare | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-1 |
| Fri 6 Aug 2021 | Away | Camelon v Preston Athletic | League Conference B | Little Kerse, Grangemouth | 1-2 |
| Fri 13 Aug 2021 | Home | Preston Athletic v Glenrothes | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 3-3 |
| Fri 27 Aug 2021 | Home | Preston Athletic v Penicuik Athletic | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | P-P |
| Mon 30 Aug 2021 | Home | Preston Athletic v Edinburgh College | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 4-0 |
| Fri 3 Sept 2021 | Away | Musselburgh Athletic v Preston Athletic | League conference B | Pinkie Playing Fields, Musselburgh | 2-1 |
| Fri 10 Sept 2021 | Home | Preston Athletic v Newtongrange Star | Eastern League Cup R1 | Middleshot 3G, Prestonpans, EH32 9RQ | 2-1 |
| Fri 17 Sept 2021 | FREE WEEK | League Conference B | |||
| Fri 24 Sept 2021 | Away | Heriot Watt University v Preston Athletic | League Conference B | John Brydson Arena, Riccarton | 8:00 |
| Fri 1 Oct 2021 | Home | Preston Athletic v Armadale Thistle | League Conference B | Pennypit Park, Prestonpans | 8:00 |
| Fri 8 Oct 2021 | Away | Edinburgh South v Preston Athletic | League Conference B | Saughton 3G, Edinburgh | 8:00 |
| Fri 15 Oct 2021 | Home | Preston Athletic v Edinburgh United | League Conference B | Pennypit Park, Prestonpans | 8:00 |
| Fri 22 Oct 2021 | Away | Sauchie v Preston Athletic | League Conference B | Lornshill Academy, Alloa | 8:00 |
| Fri 29 Oct 2021 | TBC | Eastern Knockout Cup R1 | |||
| Fri 5 Nov 2021 | Home | Preston Athletic v Kennoway Star Hearts | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 12 Nov 2021 | Away | Whitehill Welfare v Preston Athletic | League Conference B | Peffermill 3G, Edinburgh | 8:00 |
| Fri 19 Nov 2021 | Home | Preston Athletic v Camelon | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 26 Nov 2021 | Away | Glenrothes v Preston Athletic | League Conference B | Locality Hub, New Bayview, Methil | 8:00 |
| Fri 3 Dec 2021 | Away | Edinburgh College v Preston Athletic | League Conference B | John Brydson Arena, Riccarton, Edinburgh | 8:00 |
| Fri 10 Dec 2021 | Away | Penicuik Athletic v Preston Athletic | League Conference B | Peffermill 3G, Edinburgh | |
| Fri 17 Dec 2021 | TBC | Eastern League Cup R2 | |||
| Fri 24 Dec 2021 | CHRISTMAS BREAK | ||||
| Fri 7 Jan 2022 | TBC | Eastern Knockout Cup R2 | |||
| Fri 14 Jan 2022 | Home | Preston Athletic v Musselburgh Athletic | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 21 Jan 2022 | Away | Edinburgh University v Preston Athletic | League Conference B | Peffermill 3G, Edinburgh | 8:00 |
| Fri 28 Jan 2022 | Home | Preston Athletic v Heriot Watt University | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 4 Feb 2022 | Away | Armadale Thistle v Preston Athletic | League Conference B | Volunteer Park, Armadale | 8:00 |
| Fri 11 Feb 2021 | Home | Preston Athletic v Edinburgh South | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 18 Feb 2022 | Away | Edinburgh United v Preston Athletic | League Conference B | Meggetland 3G, Edinburgh | 8:00 |
| Fri 25 Feb 2022 | TBC | Easter League Cup R3/KO Cup R3 | |||
| Fri 4 Mar 2022 | Home | Preston Athletic v Sauchie | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 11 Mar 2022 | TBC | Combined Challenge Cup R1 |