strong>Pennypit Park, Prestonpans, EH32 9BN8:00
| Fri 30 July 2021 | Home | Preston Athletic v Whitehill Welfare | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 1-1 |
| Fri 6 Aug 2021 | Away | Camelon v Preston Athletic | League Conference B | Little Kerse, Grangemouth | 1-2 |
| Fri 13 Aug 2021 | Home | Preston Athletic v Glenrothes | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 3-3 |
| Fri 27 Aug 2021 | Home | Preston Athletic v Penicuik Athletic | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | P-P |
| Mon 30 Aug 2021 | Home | Preston Athletic v Edinburgh College | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 4-0 |
| Fri 3 Sept 2021 | Away | Musselburgh Athletic v Preston Athletic | League conference B | Pinkie Playing Fields, Musselburgh | 2-1 |
| Fri 10 Sept 2021 | Home | Preston Athletic v Newtongrange Star | League Cup East R1 | Middleshot 3G, Prestonpans, EH32 9RQ | 2-1 |
| Fri 17 Sept 2021 | FREE WEEK | ||||
| Fri 24 Sept 2021 | Away | Heriot Watt University v Preston Athletic | League Conference B | John Brydson Arena, Riccarton | 1-3 |
| Fri 1 Oct 2021 | Home | Preston Athletic v Armadale Thistle | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 8-0 |
| Fri 8 Oct 2021 | Away | Edinburgh South v Preston Athletic | League Conference B | Saughton 3G, Edinburgh | 4-11 |
| Fri 15 Oct 2021 | Home | Preston Athletic v Edinburgh United | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 3-2 |
| Fri 22 Oct 2021 | Away | Sauchie v Preston Athletic | League Conference B | Lornshill Academy, Alloa | 0-2 |
| Fri 29 Oct 2021 | Home | Preston Athletic v Edinburgh University | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 2-0 |
| Fri 5 Nov 2021 | Home | Preston Athletic v Kennoway Star Hearts | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Fri 12 Nov 2021 | Away | Whitehill Welfare v Preston Athletic | League Conference B | Peffermill 3G, Edinburgh | 4-6 |
| Fri 19 Nov 2021 | Home | Preston Athletic v Camelon | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Fri 26 Nov 2021 | Away | Glenrothes v Preston Athletic | League Conference B | Locality Hub, New Bayview, Methil | P-P |
| Fri 3 Dec 2021 | Away | Edinburgh College v Preston Athletic | League Conference B | Whittinghame Road, Haddington | 1-7 |
| Fri 10 Dec 2021 | Away | Penicuik Athletic v Preston Athletic | League Conference B | Peffermill 3G, Edinburgh | 1-9 |
| Fri 17 Dec 2021 | Away | Dunipace v Preston Athletic | League Cup East R2 | Westfield Park, Denny | 2-6 |
| Fri 24 Dec 2021 | CHRISTMAS BREAK | ||||
| Fri 7 Jan 2022 | Home | East Fife v Preston Athletic | Knockout Cup East R2 | Pennypit Park, Prestonpans, EH32 9BN | 3-5 |
| Fri 14 Jan 2022 | Away | Musselburgh Athletic v Preston Athletic | League Conference B | Pinkie 3G, Musselburgh | 2-0 |
| Fri 21 Jan 2022 | Away | Edinburgh University v Preston Athletic | League Conference B | Peffermill 3G, Edinburgh | 2-3 |
| Fri 28 Jan 2022 | Home | Preston Athletic v Heriot Watt University | League Conference B | Pennypit Park, Prestonpans, EH32 9RQ | 7-0 |
| Fri 4 Feb 2022 | Away | Armadale Thistle v Preston Athletic | League Conference B | Armadale Academy 3G, Armadale | 0-3 |
| Fri 11 Feb 2021 | Home | Preston Athletic v Edinburgh South | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 2-0 |
| Fri 18 Feb 2022 | Away | Edinburgh United v Preston Athletic | League Conference B | Meggetland 3G, Edinburgh | 4-4 |
| Fri 25 Feb 2022 | Home | Preston Athletic v Cumbernauld Colts | League Cup East R3 | Middleshot 3G, Prestonpans, EH32 9RQ | 3-2 |
| Fri 4 Mar 2022 | Home | Preston Athletic v Sauchie | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 2-4 |
| Fri 11 Mar 2022 | Home | Preston Athletic v Glenvale | Combined Challenge Cup R1 | Middleshot 3G, Prestonpans, EH32 9RQ | 0-1 |
| Fri 18 Mar 2022 | Away | Kennoway Star Hearts v Preston Athletic | League Conference B | Fleet Ground, Rosyth, KY11 2BA | 0-5 |
| Fri 25 Mar 2022 | Home | Preston Athletic v Kennoway Star Hearts | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | P-P |
| Fri 1 Apr 2022 | Home | Preston Athletic v Musselburgh Athletic | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 4-0 |
| Fri 8 Apr 2022 | Home | Preston Athletic v Penicuik Athletic | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 1-1 |
| Fri 15 Apr 2022 | Away | Glenrothes v Preston Athletic | League Conference B | Fleet Ground, Rosyth, KY11 2BA | 4-1 |
| Fri 22 Apr 2022 | Home | Preston Athletic v Camelon | League Conference B | Middleshot 3G, Prestonpans, EH32 9RQ | 6-0 |
| Fri 29 April 2022 | Home | Preston Athletic v Bonnyrigg Rose | League Cup East Quarter Final | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Fri 6 May 2022 | NO FIXTURE | ||||
| Fri 13 May 2022 | NO FIXTURE | ||||
| Fri 20 May 2022 | Home | Preston Athletic v Kennoway Star Hearts | League Conference B | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |