| Friday 19 Aug 2016 | Home | Preston Athletic v Bonnyrigg Rose u21s | Friendly | Middleshot 3G, Prestonpans | 2-3 |
| Friday 26 Aug 2016 | Away | Whitehill Welfare v Preston Athletic | Lowland & EoS u20s Dev Qualifying League Section A | Lasswade 3G | 5-1 |
| Friday 2 Sept 2016 | Home | Preston Athletic v BSC Glasgow | Lowland & EOS u20s Development League | Middleshot 3G, Prestonpans | 1-0 |
| Tues 6 Sept 2016 | Home | Preston Athletic v Cumbernauld Colts | Lowland & EOS u20s Dev Qualifying League Section A | Pennypit Park, Prestonpans | 1-4 |
| Friday 9 Sept 2016 | Away | Edusport Academy v Preston Athletic | Lowland & EOS u20s Dev Qualifying League Section A | Pennypit Park, Prestonpans | 1-1 |
| Thursday 15 Sept 2016 | Home | Preston Athletic v Lothian Thistle HV | Lowland &EOS u20s Dev Qualifying League Section A | Pennypit Park, Prestonpans | 1-0 |
| Sunday 25 Sept 2016 | Away | Edinburgh City v Preston Athletic | Little Big Shot Scottish Youth Cup R2 | Meadowbank, Edinburgh | 3-1 |
| Friday 30 Sept 2016 | Away | East Kilbride v Preston Athletic | Lowland & EOS u20s Dev Qualifying League Section A | K Park 3G, East Kilbride | 2-1 |
| Friday 7 Oct 2016 | Home | Preston Athletic v Spartans | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 2-6 |
| Friday 14 Oct 2016 | Away | Tynecastle v Preston Athletic | Lowland & EOS u20s Development League | Saughton 3G, Edinburgh | 2-1 |
| Friday 21 Oct 2016 | Home | Preston Athletic v Heriot-Watt University | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 1-3 |
| Friday 28 Oct 2016 | Away | Cumbernauld Colts v Preston Athletic | Lowland & EoS u20s Development League |
Broadwood Stadium, Cumbernauld | 0-3 |
| Friday 4 Nov 2016 | Home | Preston Athletic v University of Stirling | Lowland & EOS u20s Development League | Middleshot 3G, Prestonpans | 1-3 |
| Monday 14 Nov 2016 | Home | Preston Athletic v Edinburgh City | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 0-3 |
| Friday 18 Nov 2016 | Home | Preston Athletic v Edinburgh University | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 0-3 |
| Friday 25 Nov 2016 | Away | Edusport Academy v Preston Athletic | Lowland & EOS u20s Development League | Ainslie Park 3G, Edinburgh | 0-1 |
| Friday 2 Dec 2016 | Home | Preston Athletic v Lothian Thistle HV | Lowland & EOS u20s Development League | Middleshot 3G, Prestonpans | 4-4 |
| Friday 9 Dec 2016 | Away | Whitehill Welfare v Preston Athletic | Lowland & EOS u20s Development League | Lasswade 3G | 7-0 |
| Friday 16 Dec 2016 | FREE WEEK | ||||
| CHRISTMAS BREAK | |||||
| Wednesday 4 Jan 2017 | Home | Preston Athletic v Leith Athletic u21s | Friendly | Pennypit Park | 0-1 |
| Friday 13 Jan 2017 | Away | BSC Glasgow v Preston Athletic | Lowland & EOS u20s Development League | Scotstoun Leisure Centre 4G | 0-6 |
| Friday 20 Jan 2017 | Home | Preston Athletic v East Kilbride | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 1-5 |
| Friday 27 Jan 2017 | Away | Spartans v Preston Athletic | Lowland & EOS u20s Development League | Ainslie Park 3G, Edinburgh | 0-2 |
| Friday 3 Feb 2017 | Home | Preston Athletic v Tynecastle | Lowland & EOS u20s Development League | Middleshot 3G, Prestonpans | 0-6 |
| Monday 13 Feb 2017 | Away | Heriot-Watt University v Preston Athletic | Lowland & EOS u20s Development League | Riccarton 3G, Edinburgh | 4-1 |
| Friday 17 Feb 2017 | Home | Preston Athletic v Whitehill Welfare | Lowland & EOS u20s Development Challenge Cup R1 1st Leg | Middleshot 3G, Prestonpans | 3-0 |
| Friday 24 Feb 2017 | Away | Whitehill Welfare v Preston Athletic | Lowland & EOS u20s Development Challenge Cup R1 2nd Leg | Lasswade 3G | 0-8 |
| Friday 3 Mar 2017 | Home | Preston Athletic v Cumbernauld Colts | Lowland & EOS u20s Development League | Middleshot 3G, Prestonpans | 2-2 |
| Friday 10 Mar 2017 | Away | University of Stirling v Preston Athletic | Lowland & EOS u20s Development League | Gannochy Sports Club | 6-4 |
| Friday 17 Mar 2017 | Home | Edinburgh City v Preston Athletic | Lowland & EOS u20s Development League | Middleshot 3G, Prestonpans | 4-3 |
| Friday 24 Mar 2017 | Away | Edinburgh University v Preston Athletic | Lowland & EOS u20s Development League | Peffermill 3G, Edinburgh | 3-1 |
| Mon 27 Mar 2017 | Home | Preston Athletic v University of Edinburgh | Lowland & EOS u20s Development Knockout Cup R1 | Pennypit Park, Prestonpans | 0-7 |
| Friday 31 Mar 2017 | Home | Preston Athletic v Edinburgh University | Lowland & EOS u20s Development Challenge Cup R2 1st Leg | Middleshot 3G, Prestonpans | 1-4 |
| Friday 7 Apr 2017 | Away | Edinburgh University v Preston Athletic | Lowland & EOS u20s Development Challenge Cup R2 2nd Leg | Peffermill, Edinburgh | 2-1 |
| EASTER BREAK | |||||
| Friday 21 Apr 2017 | No Game | ||||
| Friday 28 Apr 2017 | Home | Preston Athletic v Edusport Academy | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 1-2 |
| Mon 1 May or Tue 2 May 2017 | Lowland & EOS u20s Development Challenge Cup Semi-Final 1st Leg | ||||
| Friday 5 May 2017 | Away | Lothian Thistle HV v Preston Athletic | Lowland & EOS u20s Development League | Riccarton 3G, Edinburgh | 5-1 |
| Mon 8 May or Tue 9 May 2017 | Lowland & EOS u20s Development Challenge Cup Semi-Final 2nd Leg | ||||
| Friday 12 May 2017 | Home | Preston Athletic v Whitehill Welfare | Lowland & EOS u20s Development League | Pennypit Park, Prestonpans | 7:45pm |
| Mon 15 May or Tue 16 May 2017 | Lowland & EOS u20s Development Knockout Cup Semi-Final | ||||
| Friday 19 May 2017 | FREE WEEK | ||||
| Mon 22 May or Tue 23 May or Fri 26 May 2017 | Lowland & EOS u20s Development Challenge Cup Final | ||||
| Fri 26 May or Fri 2 June 2017 | Lowland & EOS u20s Development Knockout Cup Final | ||||