| Fri 6 November 2020 | Home | Preston Athletic v Spartans | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | P-P |
| Fri 13 November 2020 | Away | Musselburgh Athletic v Preston Athletic | League Conference A | Pinkie 3G, Musselburgh | 8:00 |
| Fri 20 November 2020 | Home | Preston Athletic v Edinburgh South | League Conference A | Middleshot 3G, Prestonpans, EH32 9RQ | 8:00 |
| Fri 27 November 2020 | Away | Bonnyrigg Rose v Preston Athletic | League Conference A | Poltonhall 3G, Bonnyrigg | 8:00 |
| Fri 4 Dec 2020 | Home | Preston Athletic v Penicuick Athletic | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 11 Dec 2020 | Away | Gala Fairydean Rovers v Preston Athletic | League conference A | South Queensferry Sports Hub | 8:00 |
| Fri 18 Dec 2020 | Home | Preston Athletic v Glenrothes | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 8 Jan 2021 | Home | Preston Athletic v Edinburgh City | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 15 Jan 2021 | Away | Kirkcaldy & Dysart v Preston Athletic | League Conference A | Windmill Community Campus, Kirkcaldy | 8:00 |
| Fri 22 Jan 2021 | Home | Preston Athletic v Sauchie | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 29 Jan 2021 | Away | Edinburgh United v Preston Athletic | League Conference A | Meggetland 3G, Edinburgh | 8:00 |
| Fri 5 Feb 2021 | Home | Preston Athletic v Kennoway Star Hearts | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 12 Feb 2021 | Away | Edinburgh University v Preston Athletic | League Conference A | Peffermill 3G, Edinburgh | 8:00 |
| Fri 19 Feb 2021 | Home | Preston Athletic v Camelon | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |
| Fri 26 Feb 2021 | Away | Kelty Hearts v Preston Athletic | League Conference A | New Central Park, Kelty | 8:00 |
| Fri 5 Mar 2021 | Away | Spartans v Preston Athletic | League Conference A | Ainslie Park 3G, Edinburgh | 8:00 |
| Fri 12 Mar 2021 | Home | Preston Athletic v Musselburgh Athletic | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 19 Mar 2021 | Away | Edinburgh South v Preston Athletic | League Conference A | Saughton 3G, Edinburgh | 8:00 |
| Fri 26 Mar 2021 | Home | Preston Athletic v Bonnyrigg Rose | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |
| Fri 9 Apr 2021 | Away | Penicuik Athletic v Preston Athletic | League Conference A | Peffermill 3G, Edinburgh | 8:00 |
| Fri 16 Apr 2021 | Home | Preston Athletic v Gala Fairydean Rovers | League Conference A | Pennypit Park, Prestonpans | 8:00 |
| Fri 23 Apr 2021 | Away | Glenrothes v Preston Athletic | League Conference A | Locality Hub, Bayview Stadium, Methil | 8:00 |
| Fri 30 Apr 2021 | Away | Edinburgh City v Preston Athletic | League Conference A | Ainslie Park 3G, Edinburgh | 8:00 |
| Mon 3 May 2021 | Away | Camelon v Preston Athletic | League Conference A | Little Kerse, Grangemouth | 8:00 |
| Fri 7 May 2021 | Home | Preston Athletic v Kirkcaldy & Dysart | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |
| Fri 14 May 2021 | Away | Sauchie v Preston Athletic | League Conference A | Lornshill Academy, Alloa | 8:00 |
| Fri 21 May 2021 | Home | Preston Athletic v Edinburgh United | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |
| Fri 28 May 2021 | Away | Kennoway Star Hearts v Preston Athletic | League Conference A | Treaton Park, Star of Markinch | 8:00 |
| Mon 31 May 2021 | Home | Preston Athletic v Kelty Hearts | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |
| Fri 4 Jun 2021 | Home | Preston Athletic v Edinburgh University | League Conference A | Pennypit Park, Prestonpans, EH32 9BN | 8:00 |